We recommend storing basecalled FASTQ and/or BAM files, which will allow the use of standard downstream analysis tools.

Storing .POD5 files with raw read data is optional. This will permit re-basecalling of data when new algorithms are released by Oxford Nanopore or third parties. In some cases, new releases of basecallers have enabled improvements in basecalling accuracy, modified basescalling, reference-guided SNP calling, or polishing of data.