Oxford Nanopore Technologies

There are multiple ways to demultiplex barcoded reads generated by Nanopore sequencing.

When starting a run in MinKNOW with live basecalling enabled, you can select the barcoding kits you have used and the software will separate your reads into barcoded folders containing the fast5 and fastq files.

In addition, MinKNOW offers a post-run demultiplexing function as described <a href="https://community.nanoporetech.com/protocols/experiment-companion-minknow/v/mke_1013_v1_revbd_11apr2016/post-run-barcoding?from=support" rel="nofollow noopener noreferrer" target="_blank">here</a>.

Stand-alone Guppy may also be used from the command line to demultiplex after sequencing. Please refer to <a href="http://help.nanoporetech.com/en/articles/6628059-how-do-i-use-guppy-to-demultiplex-my-barcoded-reads">How do I use Guppy to demultiplex my barcoded reads?</a> or <a href="https://community.nanoporetech.com/protocols/Guppy-protocol/v/gpb_2003_v1_revt_14dec2018/barcoding-demultiplexing?from=support" rel="nofollow noopener noreferrer" target="_blank">the Guppy protocol</a> for more details.

How can I demultiplex barcoded samples?

Find answers and get help from Intercom Support and Community Experts

Empty Help Center

Uh oh. That page doesn’t exist.

Disappointed

Neutral

Smiley

Title

Track the progress of all tickets related to your company.

Tickets portal.

{assigneeName} needs more information from you