You can determine the number of reads that are being rejected due to adaptive sampling via two methods:

1. During the run: using the read length histogram in MinKNOW by ticking the box "Split by read end reason".

If you are running in a mode where a sufficient number of reads should be rejected to produce a peak of short reads, there should be a bimodal distribution with a peak at a base-count much lower than what you expect from the sample.

The reads rejected by adaptive sampling will have the label "Adaptive sampling voltage reversal"

2. Immediately after the sequencing run starts: please ensure that an adaptive_sampling.csv file was initialized into the "other_reports" folder.

This file will contain information about the final decision that was taken by adaptive sampling for each read.