It is necessary to always perform the end-prep step in order to prepare the DNA for adapter ligation.
Even in cases with a PCR step using a polymerase that adds an A-tail, the end-prep is still necessary.
It is necessary to always perform the end-prep step in order to prepare the DNA for adapter ligation.
Even in cases with a PCR step using a polymerase that adds an A-tail, the end-prep is still necessary.