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Do I have to use SPRI beads for the purification step during library prep?
Do I have to use SPRI beads for the purification step during library prep?
Updated over a week ago

Although alternative purification methods can be used post fragmentation and end-repair/dA tailing step, we strongly suggest using SPRI beads (the motor protein will not withstand exposure to the guanidinium chloride present in some kit buffers).

Using different SPRI beads-to-sample ratios allows fragment size selection (here you can see an example). For more information on different extraction methods and size selection check this info sheet here.

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