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Why do we use two pools of probes instead of just one on the tiling method?
Why do we use two pools of probes instead of just one on the tiling method?
Updated over a week ago

The tiling method or “Bricklayer” tiling approach is recommended when the region of interest (ROI) is >20 kb. Two sets of probes are designed to break up the ROI into overlapping sections, ensuring good coverage of the entire length of ROI.

Cas9 tiling approach

Please see the Targeted, amplification-free DNA sequencing using CRISPR/Cas info sheet for more details.

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