All Collections
Library prep
Cas9
What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing?
What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing?
Updated over a week ago

We highly recommend performing dephosphorylation of the genomic DNA step (~15 min) to remove the 5’ phosphates from the ends of the genomic DNA.

This step will help reducing the ligation of sequencing adapters to non-target DNA therefore background reads will be heavily reduced.

Did this answer your question?