What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing? | Oxford Nanopore Technologies Help Center

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What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing?

What’s the purpose of the dephosphorylation of the genomic DNA in Cas9 targeted sequencing?

Updated over a week ago

We highly recommend performing dephosphorylation of the genomic DNA step (~15 min) to remove the 5’ phosphates from the ends of the genomic DNA.

This step will help reducing the ligation of sequencing adapters to non-target DNA therefore background reads will be heavily reduced.