All Collections
Library prep
What should I do if a white precipitate appears when adding the adapter ligation mix to the dA-tailed DNA?
What should I do if a white precipitate appears when adding the adapter ligation mix to the dA-tailed DNA?
Updated over a week ago

A white precipitate may form upon addition of the adapter ligation mix to the dA-tailed DNA. Adding the ligation mixture in two parts helps to reduce precipitation.

However, the presence of a precipitate does not necessarily indicate failure of ligation of the sequencing adapter to target molecule ends.

Formation of the precipitate is likely due to the large amount of DNA used in the experiment. Users can proceed with the rest of the experiment.

Did this answer your question?