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How is enrichment of the Region of Interest (ROI) achieved through the Cas9 targeted sequencing experiment?
How is enrichment of the Region of Interest (ROI) achieved through the Cas9 targeted sequencing experiment?
Updated over a week ago

Upon loading of the Cas9 ribonucleoprotein particles (RNPs) onto the Region of Interest (ROI), cleavage takes place at the 5โ€™ end of the target strand, while, most of the time, the Cas9 RNP remains bound at the PAM-distal end.

As a result, the end of the ROI becomes exposed and thus available for ligation with the sequencing adapter during library prep, whereas the Cas9-bound end is protected and unavailable for ligation.

More details are available here.

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