All Collections
Library prep
Cas9
How does Cas9 targeted sequencing work?
How does Cas9 targeted sequencing work?
Updated over a week ago

The Cas9 targeted sequencing approach includes two parts, 1. targeting by CRISPR/Cas9-mediated sequence-specific cleavage of the region of interest (ROI) and 2. sequencing of ROI using the ligation sequencing approach.

Additional steps are included to increase the sequencing specificity of the ROI. The diagram below illustrates each step involved. A detailed protocol can be found here.

Cas9 protocol scheme
Did this answer your question?