The RNA integrity number is a quality control test that measures the level of degradation of the RNA in the extract on a scale from 1 to 10 with 10 being the least degraded. It is recommended that the extract have a RIN of 7 or higher before proceeding with library prep.
Figure 1. The length distribution of reads that align to the target organism (*S. cerevisiae*) for samples with different RIN values
For more information, please visit the article RNA stability in our Nanopore know-how section.