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What are the features of the Rapid Barcoding Kit 96 (SQK-RBK114.96)?
What are the features of the Rapid Barcoding Kit 96 (SQK-RBK114.96)?
Updated over a week ago

The Rapid Barcoding Kit 96 (SQK-RBK114.96) contains 96 barcodes for rapid attachment to the input DNA in the same way as used in the Rapid Barcoding Kit 96 (SQK-RBK110.96)

This kit has been updated to use our newest Kit 14 Q20+ chemistry which includes improved modal raw read sequencing accuracies with higher output on our latest nanopore: R10.4.1. The flow cell priming and sequencing reagents are also included as part of the sequencing kit and have been reformulated to be compatible with the Kit 14 chemistry upgrade and R10.4.1 nanopore.

Kit 14 also includes previous updates such as the higher capture rate of DNA to enable lower flow cell loading amounts, and fuel fix technology, allowing users to run longer experiments without the need for fuel addition during the run.

Taking advantage of the improved chemistry in SQK-RBK114.96, a more easily executable protocol has been developed for Covid sequencing - PCR tiling of SARS-CoV-2 virus - Rapid Barcoding Kit 96 V14 and Midnight RT PCR Expansion (SQK-RBK114.96 and EXP-MRT001).

In addition, as with SQK-RBK110.96, SQK-RBK114.96 is suitable for long input DNA fragments, such as genomic DNA, as described in the Rapid Barcoding Kit 96 (SQK-RBK114.96) protocol.

More details on the SQK-RBK114.96 kit can be found in the release note.

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