The Ultra-Long DNA Sequencing Kit (SQK-ULK114) is recommended for users who work with ultra-high molecular weight (uHMW) DNA and would like read lengths with an N50 >50 kb.
The end-to-end workflow enables users to extract and sequence their sample in just one day:
- uHMW gDNA extraction using the NEB Monarch® HMW DNA Extraction Kit for Tissue
- Library prep using the SQK-ULK114 kit which is based on transposase chemistry - the transposase simultaneously cleaves template molecules and attaches tags to the cleaved ends, and then the Rapid Sequencing Adapters are added to the tagged ends.
We continuously validate various input samples for this protocol and will share our results as Community posts, such as Updated Ultra-Long DNA Sequencing Kit protocol and size selection of HMW DNA.